Gel Edos and you can Advertisement had been counted having fun with Estradiol EIA System (Cayman Chemicals, MI) and androstenedione chemical immunoassay kit (Oxford Biomedical Lookup Inc., MI), correspondingly. ELISAs of T and you will E1 were did with respect to the exact same procedure of 17,20?-P (Matsuyama ainsi que al., 1998b) and you will described below temporarily.
Stock solutions: Coating buffer, 0.05 M carbonate buffer pH 8.4, containing 0.05% NaNstep three; washing solution, 0.85% NaCl; blocking solution, 0.05 M PBS containing 0.1% BSA, 3% sucrose, and 0.005% thimerosal; assay buffer, 0.05 M borate buffer, pH 7.8, containing 0.1% BSA and 0.01% thimerosal; substrate solution, 0.2 M citrate buffer, pH 4.5, containing 0.01% H2O2, added 0.5% o-phenylenediamine immediately before use; stopping solution, 6 N H2SO4. Continue reading “In the vitro hair follicle incubation having radiolabeled steroid precursors”