So you’re able to image countries most often active in the hereditary control over leaf rust opposition, the latest QTL-review occurrence index (Chardon et al. 200cuatro) is computed (Fig. step three). So it statistic increases within the a region having (i) what amount of QTL of other experiments, (ii) this new proximity between QTL ranks, and you will (iii) the accuracy of the individual QTL status quote.
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Fig. step 3. The review fact try determined every 1 centimorgan (cM) and you may plotted along for each and every chromosome (bluish bend). Two horizontal dotted outlines will be mediocre property value the newest figure (0.055) as well as the threshold having higher thinking (0.2seven5), identified as 5 times the common worthy of (Chardon mais aussi al. 2004). Bars below the assessment curve represent the fresh wheat chromosomes in which QTL were estimated. Light gray (green) packages depict meta-QTL harboring at the least dos QTL, while dark-gray (red) boxes show unique QTL. Positions regarding previously mapped Lr genes when you look at the wheat is actually depicted because of the triangles below chromosome bars; Lr genes colocalized having meta-QTL is conveyed inside deep gray (blue).
Brand new evaluation index is calculated most of the centimorgan and you will estimates had been obtained for every of one’s 144 estimated QTL (Fig. 3). In total, 52 analysis peaks have been gotten, of which 47 surpassed the average property value brand new statistic (0.055) across the genome and you will portrayed genomic beautiful spots having leaf corrosion resistance. Of your 47 highs, twenty-five demonstrated philosophy surpassing new large-well worth threshold (0.275) (Fig. 3). The number of peaks surpassing the average tolerance ranged in one from inside the chromosome 4A and you will 7A to four inside chromosomes 1B, 2D, and 7B. To the latter, all of the four highs in addition to surpassed new higher-well worth endurance.
QTL meta-investigation.
The 144 QTL projected onto the Grain Chemical 2004 chart had been confronted with meta-study making use of the qualities from Goffinet and you can Gerber (2000) if the amount of QTL in an effective chromosome is actually lower than ten and people regarding Veyrieras ainsi que al. (2007) in the event the number are ten or more. After the an AIC, 141 QTL was in fact grouped into the 48 MQTL (Desk dos). The software didn’t consider around three QTL to possess meta-analysis for their lower LOD rating viewpoints otherwise higher Au moment ou. In every, thirty-five MQTL, spanning 128 QTL, was the result of the fresh new consolidation of at least 2 QTL, and you will 13 QTL remained once the book QTL (Desk 2).
a the higher the main dining table suggests MQTL comprising during the minimum a couple of clustered QTL in addition to down region shows QTL kept since the unique QTL.
c AUDPC = area according to the problem advances bend, DS = condition seriousness, EA = early aborted colonies, EST = created colonies, Web browser = illness performance, It = illness type of, LIA = leaf infected city, LP = latent several months, LR = leaf resistance, LS = lesion size, LTN = leaf tip necrosis, NEC = necrotic colonies, SPL = spore production for every single lesion, and you can SPS = spore design for every single tool out of sporulating tissue.
| Chromosome | MQTL | Position (cM) | Si (95%) | Flanking indicators | QTL (n) b | Feature c | Opposition source |
|---|---|---|---|---|---|---|---|
| 1A | MQTL1 | Xfba254–Xwmc95b | 4 | AUDPC, DS | Apache, Sujata | ||
| 1B | MQTL2 | step one.8six | Xcdo98.2–Xmwg837b | 6 | AUDPC, DS, It, LTN | Forno, MG5323, Oberkulmer, Pastor, Sujata | |
| MQTL3 | 8.46 | Xbarc81–Xfba294 | 2 | AUDPC, DS | Bainong 64 | ||
| MQTL4 | dos.10 | Xfba266c–XksuI27 | 2 | DS | Pastor, Pavon 76 | ||
| MQTL5 | 0.28 | XksuI27–Xbarc80 | 2 | AUDPC, DS | Francolin#step 1 | ||
| 2A | MQTL6 | cuatro.62 | Xglk554–Xcmwg682b | 2 | AUDPC | Colosseo, Forno | |
| 2B | MQTL7 | step three.68 | Xgwm55–Xgwm429 | 4 | AUDPC, DS, They | Carberry, Kariega, Avocet S, W-7984 | |
| MQTL8 | Xwmc265–Xgwm877.step 1 | 7 | AUDPC, DS, LP | Apache, Creso | |||
| 2D | MQTL9 | 9.thirty-two | Xgwm455–Xbcd718 | 3 | AUDPC, DS | Harmony, Forno, MN99394-step one | |
| MQTL10 | 8.forty | Xfba74a–Xgwm30.1 | 3 | AUDPC, DS, LTN | Forno, Pastor | ||
| MQTL11 | 4.eleven | Xcdo1008–Xksud23a | 6 | AUDPC, DS, SPS | Balance | ||
| 3A | MQTL12 | 6.73 | Xcdo1345–Xgwm2 | 2 | LR, LTN | Forno, Oberkulmer | |
| MQTL13 | Xcfa2134–XgbxG242 | 2 | It, LIA | TA4152-60 | |||
| MQTL14 | 2.32 | Xmwg570–Xcdo113 | 2 | AUDPC, LIA | Lloyd | ||
| 3B | MQTL15 | dos.08 | XksuG30a–Xgwm533a | 9 | DS, Web browser, It, LIA, LP, SPL | Equilibrium, Capo, Opata 85 | |
| MQTL16 | 0.89 | Xgwm566–Xgwm888.step three | 3 | DS, They, LIA | Francolin#1, TA4152-60 | ||
| 4A | MQTL17 | XgbxR247–Xpsr593.2 | 2 | DS, They | Opata 85 | ||
| 4B | MQTL18 | Xgwm495a–Xcsu25 | 3 | AUDPC, DS | Avocet S, Creso, Forno | ||
| MQTL19 | Xgwm131.2–Xmwg11a | 2 | DS, LTN | Carberry, Oberkulmer | |||
| 4D | MQTL20 | 2.fifty | Xbarc98–Xfbb336 | 3 | AUDPC, It, LIA | Sujata, TA4252-sixty | |
| MQTL21 | Xcdo1312a–Xfba177b | 4 | AUDPC, DS, LS, SPL | Balance | |||
| 5B | MQTL22 | 4.79 | Xbarc4–Xgwm371 | 2 | AUDPC, DS | Kariega, SHA3/CBRD | |
| MQTL23 | 8.83 | Xpsp3065–Xgwm118 | 5 | It, LIA, LTN | Americano 25e, Capo, Carberry, TA4152-60 | ||
| 6A | MQTL24 | Xmwg67.1–XksuH4.2 | 6 | AUDPC, DS, LP | Balance | ||
| 6B | MQTL25 | S25M19_7–Xcdo476.dos | 2 | AUDPC, DS | Bainong 64 | ||
| MQTL26 | Gpc-B1–Xwmc388a | 2 | AUDPC, DS | Bainong 65 | |||
| MQTL27 | Xgwm133b–Xfbb130 | 2 | DS, They | Pavon 76, TA4152-60 | |||
| MQTL28 | 6.91 | Xbarc134–Xwmc417b | 2 | AUDPC, DS | Kariega, Pastor | ||
| 7A | MQTL29 | 4.73 | XWC2028g–P78/M49.4 | 5 | AUDPC, DS | Balance | |
| 7B | MQTL30 | 3.58 | Xwmc335–Xgwm68.dos | 5 | AUDPC, DS, LIA | Colosseo, Forno, Kariega, SHA3/CBRD | |
| MQTL31 | dos.17 | S25M15_19–Xgwm112 | 4 | DS, They, LTN | MG5323, Oberkulmer | ||
| MQTL32 | cuatro.42 | Xwmc273c–Xwmc311 | 4 | AUDPC, DS, LTN | Forno, Oberkulmer, Opata 85, Pastor | ||
| MQTL33 | 0.53 | Xgwm577–Xbarc340.2 | 11 | AUDPC, DS, EA, EST, LIA, LP, NEC | Colosseo, Creso, Sujata | ||
| 7D | MQTL34 | step 1.75 | Xbcd1438–Xcnl6b | 3 | AUDPC, DS, LTN | Forno, MN98550-5 | |
| MQTL35 | nine.39 | Xgwm44–Xcfa2174c | 2 | DS, LTN | Oberkulmer, Opata 85 | ||
| 1A | … | 123.5 | Xwmc237–XksuE11.1a | 1 | DS | SHA3/CBRD | |
| 1B | … | six.thirteen | XksuF43.1–Xabc155c | 1 | LIA | Capo | |
| 2A | … | Xfbb329–Xgwm339 | 1 | LP | Creso | ||
| … | step three.09 | Xfbb353.2–Xglk554 | 1 | LIA | Colosseo | ||
| 2B | … | 4.98 | Xwmc344–XksuD22 | 1 | LIA | Capo | |
| … | cuatro.twenty seven | Xfba310.1–Xbcd1231 | 1 | LIA | Capo | ||
| 2D | … | Xwmc243–Xgwm608.3 | 1 | DS | SHA3/CBRD | ||
| 5A | … | dos.00 | 5.55 | Xcnl6b–Xgwm154a | 1 | LTN | Oberkulmer |
| … | Xbcd508–Xfbb255x | 1 | DS | Pastor | |||
| … | Xwg114a–P43/M62-5 | 1 | EA | Creso | |||
| 6A | … | 9.88 | Sr26–P38/M51-cuatro | 1 | LTN | Oberkulmer | |
| 7D | … | 3.forty-eight | XgbxG564–Yr18 | 1 | DS | Opata 85 | |
| … | Xgwm121–Xbarc53 | 1 | LTN | Carberry |